The expression and clinical significance of microRNA-34a in diffuse lager B-cell lymphoma

This study aims to investigate the role and mechanism of miR-34a in diffuse large B-cell lymphoma (DLBCL). Totally 30 DLBCL patients and 30 reactive lymph node hyperplasia patients were selected as DLBCL group and control group respectively. Lymph node tissues were collected. The miR-34a inhibitors or mimics were transfected into OCI-LY10 cells. RT-qPCR was performed to detect levels of miR-34a and AXL receptor tyrosine kinase (AXL) mRNA. Western Blot was used to detect protein levels of AXL. Cell migration and invasion was detected by transwell and matrigel assay. Cell proliferation was measured by MTT assay. Cell apoptosis was assessed by flow cytometry. The results showed that miR-34a level was significantly decreased in DLBCL lymph node tissue compared with that in control lymph tissue. However, the mRNA and protein levels of AXL were significantly higher in lymph tissue of DLBCL group than that in control group. In transfected OCI-LY10 cells, the over-expression of miR-34a could inhibit the mRNA and protein levels of AXL. However, the reduction of miR-34a in OCI-LY10 cells elevated the expressions of AXL mRNA and protein. Besides, the proliferation, metastasis and infiltration of OCI-LY10 cells were increased significantly while the apoptosis was decreased significantly when miR-34a level was reduced. MiR-34a may involve in the pathogenesis of DLBCL via its regulation on AXL, and miR-34a may be used as a new therapeutic target of DLBCL.